PMID:16640594

Citation	Murata, Y, Ma, JF, Yamaji, N, Ueno, D, Nomoto, K and Iwashita, T (2006) A specific transporter for iron(III)-phytosiderophore in barley roots. Plant J. 46:563-72
Abstract	Iron acquisition of graminaceous plants is characterized by the synthesis and secretion of the iron-chelating phytosiderophore, mugineic acid (MA), and by a specific uptake system for iron(III)-phytosiderophore complexes. We identified a gene specifically encoding an iron-phytosiderophore transporter (HvYS1) in barley, which is the most tolerant species to iron deficiency among graminaceous plants. HvYS1 was predicted to encode a polypeptide of 678 amino acids and to have 72.7% identity with ZmYS1, a first protein identified as an iron(III)-phytosiderophore transporter in maize. Real-time RT-PCR analysis showed that the HvYS1 gene was mainly expressed in the roots, and its expression was enhanced under iron deficiency. In situ hybridization analysis of iron-deficient barley roots revealed that the mRNA of HvYS1 was localized in epidermal root cells. Furthermore, immunohistological staining with anti-HvYS1 polyclonal antibody showed the same localization as the mRNA. HvYS1 functionally complemented yeast strains defective in iron uptake on media containing iron(III)-MA, but not iron-nicotianamine (NA). Expression of HvYS1 in Xenopus oocytes showed strict specificity for both metals and ligands: HvYS1 transports only iron(III) chelated with phytosiderophore. The localization and substrate specificity of HvYS1 is different from those of ZmYS1, indicating that HvYS1 is a specific transporter for iron(III)-phytosiderophore involved in primary iron acquisition from soil in barley roots.
Links	PubMed Online version:10.1111/j.1365-313X.2006.02714.x
Keywords	Amino Acid Sequence; Animals; Ferric Compounds/metabolism; Hordeum/cytology; Hordeum/metabolism; Iron-Binding Proteins/metabolism; Membrane Transport Proteins/analysis; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/physiology; Molecular Sequence Data; Phylogeny; Plant Proteins/analysis; Plant Proteins/chemistry; Plant Proteins/physiology; Plant Roots/metabolism; Protein Structure, Tertiary; RNA, Messenger/metabolism; Sequence Alignment; Xenopus
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HORVU:Q2PGC4		GO:0005886: plasma membrane	ECO:0000314:		C		Figure 4	complete CACAO 8364
HORVU:Q2PGC4	part_of	GO:0005886: plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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