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Petrásek, J, Mravec, J, Bouchard, R, Blakeslee, JJ, Abas, M, Seifertová, D, Wisniewska, J, Tadele, Z, Kubes, M, Covanová, M, Dhonukshe, P, Skupa, P, Benková, E, Perry, L, Krecek, P, Lee, OR, Fink, GR, Geisler, M, Murphy, AS, Luschnig, C, Zazímalová, E and Friml, J (2006) PIN proteins perform a rate-limiting function in cellular auxin efflux. Science 312:914-8
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Arabidopsis/cytology; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Transport; Cell Membrane/metabolism; Cells, Cultured; Gravitropism; HeLa Cells; Humans; Indoleacetic Acids/metabolism; Kinetics; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutation; Naphthaleneacetic Acids/metabolism; Phthalimides/pharmacology; Plant Roots/physiology; Saccharomyces cerevisiae/genetics; Tobacco; Transfection; Transformation, Genetic
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