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PMID:16574149
| Citation |
Vadyvaloo, V, Smirnova, IN, Kasho, VN and Kaback, HR (2006) Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease. J. Mol. Biol. 358:1051-9 |
|---|---|
| Abstract |
Building a three-dimensional model of the sucrose permease of Escherichia coli (CscB) with the X-ray crystal structure lactose permease (LacY) as template reveals a similar overall fold for CscB. Moreover, despite only 28% sequence identity and a marked difference in substrate specificity, the structural organization of the residues involved in sugar-binding and H(+) translocation is conserved in CscB. Functional analyses of mutants in the homologous key residues provide strong evidence that they play a similar critical role in the mechanisms of CscB and LacY. |
| Links |
PubMed PMC2786776 Online version:10.1016/j.jmb.2006.02.050 |
| Keywords |
Amino Acid Sequence; Binding Sites/genetics; Carbohydrate Metabolism; Conserved Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Genes, Bacterial; Hydrogen/metabolism; Kinetics; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Models, Molecular; Monosaccharide Transport Proteins/chemistry; Monosaccharide Transport Proteins/genetics; Monosaccharide Transport Proteins/metabolism; Mutagenesis, Site-Directed; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Symporters/chemistry; Symporters/genetics; Symporters/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008643: carbohydrate transport |
ECO:0000315: |
P |
Figure 2 shows wild-type CscB compared to CscB mutants with amino acid residue mutations. The graph shows the concentration of sucrose transport is greater for the wild-type CscB. |
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See also
References
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