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PMID:16541108
| Citation |
Dez, C, Houseley, J and Tollervey, D (2006) Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae. EMBO J. 25:1534-46 |
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| Abstract |
We previously hypothesized that HEAT-repeat (Huntington, elongation A subunit, TOR) ribosome synthesis factors function in ribosome export. We report that the HEAT-repeat protein Sda1p is a component of late 60S pre-ribosomes and is required for nuclear export of both ribosomal subunits. In strains carrying the ts-lethal sda1-2 mutation, pre-60S particles were rapidly degraded following transfer to 37 degrees C. Polyadenylated forms of the 27S pre-rRNA and the 25S rRNA were detected, suggesting the involvement of the Trf4p/Air/Mtr4p polyadenylation complex (TRAMP). The absence of Trf4p suppressed polyadenylation and stabilized the pre-rRNA and rRNA. The absence of the nuclear exosome component Rrp6p also conferred RNA stabilization, with some hyperadenylation. We conclude that the nuclear-restricted pre-ribosomes are polyadenylated by TRAMP and degraded by the exosome. In sda1-2 strains at 37 degrees C, pre-40S and pre-60S ribosomes initially accumulated in the nucleoplasm, but then strongly concentrated in a subnucleolar focus, together with exosome and TRAMP components. Localization of pre-ribosomes to this focus was lost in sda1-2 strains lacking Trf4p or Rrp6p. We designate this nucleolar focus the No-body and propose that it represents a site of pre-ribosome surveillance. |
| Links |
PubMed PMC1440318 Online version:10.1038/sj.emboj.7601035 |
| Keywords |
Active Transport, Cell Nucleus; Cell Cycle Proteins/genetics; Cell Nucleolus/genetics; Cell Nucleolus/metabolism; DEAD-box RNA Helicases; DNA-Directed DNA Polymerase/genetics; DNA-Directed DNA Polymerase/metabolism; Mutation; Nuclear Proteins/genetics; Polyadenylation; RNA Helicases/genetics; RNA Helicases/metabolism; RNA Precursors/genetics; RNA Precursors/physiology; Ribosomes/genetics; Ribosomes/physiology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Temperature |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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