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PMID:16530043
| Citation |
Grimaud, C, Bantignies, F, Pal-Bhadra, M, Ghana, P, Bhadra, U and Cavalli, G (2006) RNAi components are required for nuclear clustering of Polycomb group response elements. Cell 124:957-71 |
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| Abstract |
Drosophila Polycomb group (PcG) proteins silence homeotic genes through binding to Polycomb group response elements (PREs). Fab-7 is a PRE-containing regulatory element from the homeotic gene Abdominal-B. When present in multiple copies in the genome, Fab-7 can induce long-distance gene contacts that enhance PcG-dependent silencing. We show here that components of the RNA interference (RNAi) machinery are involved in PcG-mediated silencing at Fab-7 and in the production of small RNAs at transgenic Fab-7 copies. In general, these mutations do not affect the recruitment of PcG components, but they are specifically required for the maintenance of long-range contacts between Fab-7 copies. Dicer-2, PIWI, and Argonaute1, three RNAi components, frequently colocalize with PcG bodies, and their mutation significantly reduces the frequency of PcG-dependent chromosomal associations of endogenous homeotic genes. This suggests a novel role for the RNAi machinery in regulating the nuclear organization of PcG chromatin targets. |
| Links |
PubMed Online version:10.1016/j.cell.2006.01.036 |
| Keywords |
Animals; Animals, Genetically Modified; Cell Nucleus/metabolism; Chromosomes/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Drosophila melanogaster/metabolism; Gene Expression Regulation, Developmental; Gene Silencing; In Situ Hybridization, Fluorescence; Larva/anatomy & histology; Larva/physiology; Mutation; Phenotype; RNA Interference; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Response Elements |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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