PMID:1652372

Citation	Moll, T, Tebb, G, Surana, U, Robitsch, H and Nasmyth, K (1991) The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell 66:743-58
Abstract	The intracellular localization of the S. cerevisiae transcription factor SWI5 is cell cycle dependent. The protein is nuclear in G1 cells but cytoplasmic in S, G2, and M phase cells. We have identified SWI5's nuclear localization signal (NLS) and show that it can confer cell cycle-dependent nuclear entry to a heterologous protein. Located within or close to the NLS are three serine residues, mutation of which results in constitutive nuclear entry. These residues are phosphorylated in a cell cycle-dependent manner in vivo, being phosphorylated when SWI5 is in the cytoplasm and dephosphorylated when it is in the nucleus. As all three serines are phosphorylated by purified CDC28-dependent H1 kinase activity in vitro, we propose a model in which the CDC28 kinase acts directly to control nuclear entry of SWI5.
Links	PubMed
Keywords	Amino Acid Sequence; Cell Compartmentation; Cell Cycle; Cell Cycle Proteins; Cell Nucleus/metabolism; Cloning, Molecular; DNA Mutational Analysis; DNA-Binding Proteins; Fungal Proteins/chemistry; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal; Histones/metabolism; Maturation-Promoting Factor/metabolism; Molecular Sequence Data; Peptide Mapping; Phosphoproteins/metabolism; Phosphorylation; Protamine Kinase/metabolism; Protein Kinases/physiology; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins; Transcription Factors/chemistry; Transcription Factors/metabolism; Transcription, Genetic
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