GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16510868
| Citation |
Chen, HB, Shen, J, Ip, YT and Xu, L (2006) Identification of phosphatases for Smad in the BMP/DPP pathway. Genes Dev. 20:648-53 |
|---|---|
| Abstract |
Phosphorylation of the SSXS motif of Smads is critical in activating the transforming growth factor beta (TGF-beta) and bone morphogenetic protein (BMP) pathways. However, the phosphatase(s) involved in dephosphorylating and hence inactivating Smads remained elusive. Through RNA interference (RNAi)-based screening of serine/threonine phosphatases in Drosophila S2 cells, we identified pyruvate dehydrogenase phosphatase (PDP) to be required for dephosphorylation of Mothers against Decapentaplegic (MAD), a Drosophila Smad. Biochemical and genetic evidence suggest that PDP directly dephosphorylates MAD and inhibits signal transduction of Decapentaplegic (DPP). We show that the mammalian PDPs are important in dephosphorylation of BMP-activated Smad1 but not TGF-beta-activated Smad2 or Smad3. Thus, PDPs specifically inactivate Smads in the BMP/DPP pathway. |
| Links |
PubMed PMC1413280 Online version:10.1101/gad.1384706 |
| Keywords |
Animals; Base Sequence; Bone Morphogenetic Proteins/metabolism; DNA Primers; Drosophila; Drosophila Proteins/metabolism; Phosphorylation; Polymerase Chain Reaction; Pyruvate Dehydrogenase (Lipoamide)-Phosphatase/metabolism; Signal Transduction; Smad Proteins/metabolism; Subcellular Fractions/enzymology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
