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PMID:16501079
| Citation |
Ryzhova, EV, Vos, RM, Albright, AV, Harrist, AV, Harvey, T and González-Scarano, F (2006) Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages. J. Virol. 80:2694-704 |
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| Abstract |
Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM. |
| Links |
PubMed PMC1395445 Online version:10.1128/JVI.80.6.2694-2704.2006 |
| Keywords |
Annexin A2/metabolism; Antigens, CD/metabolism; Antigens, CD63; Cells, Cultured; Gene Products, gag/metabolism; Genes, gag; HIV-1/metabolism; HIV-1/pathogenicity; HIV-1/physiology; Humans; Macrophages/virology; Platelet Membrane Glycoproteins/metabolism; Protein Precursors/metabolism; Virus Assembly; Virus Replication |
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Significance
Annotations
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