PMID:16484190

Citation	Sherlock, O, Dobrindt, U, Jensen, JB, Munk Vejborg, R and Klemm, P (2006) Glycosylation of the self-recognizing Escherichia coli Ag43 autotransporter protein. J. Bacteriol. 188:1798-807
Abstract	Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to this exclusive group, namely, antigen 43 (Ag43), a self-recognizing autotransporter protein. By mass spectrometry Ag43 was demonstrated to be glycosylated by addition of heptose residues at several positions in the passenger domain. Glycosylation of Ag43 by the action of the Aah and TibC glycosyltransferases was observed in laboratory strains. Importantly, Ag43 was also found to be glycosylated in a wild-type strain, suggesting that Ag43-glycosylation may be a widespread phenomenon. Glycosylation of Ag43 does not seem to interfere with its self-associating properties. However, the glycosylated form of Ag43 enhances bacterial binding to human cell lines, whereas the nonglycosylated version of Ag43 does not to confer this property.
Links	PubMed PMC1426561 Online version:10.1128/JB.188.5.1798-1807.2006
Keywords	Adhesins, Bacterial/genetics; Adhesins, Bacterial/metabolism; Amino Acid Sequence; Antigens, Bacterial/genetics; Antigens, Bacterial/metabolism; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Catalytic Domain/genetics; Catalytic Domain/physiology; Cell Line; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Glycosylation; Humans; Molecular Sequence Data
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:AG43		GO:0006486: protein glycosylation	ECO:0000314:		P		Figure 1, Figure 4B	complete CACAO 6180
ECOLX:F8SKF2		GO:0016757: transferase activity, transferring glycosyl groups	ECO:0000314:		F		Fig 1	complete CACAO 6182
ECOLX:F8SKF2	enables	GO:0016757: transferase activity, transferring glycosyl groups	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
ECOH1:TIBC		GO:0016757: transferase activity, transferring glycosyl groups	ECO:0000314:		F		Fig 1	complete CACAO 6184
ECOH1:TIBC	enables	GO:0016757: transferase activity, transferring glycosyl groups	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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