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PMID:16484190

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Citation

Sherlock, O, Dobrindt, U, Jensen, JB, Munk Vejborg, R and Klemm, P (2006) Glycosylation of the self-recognizing Escherichia coli Ag43 autotransporter protein. J. Bacteriol. 188:1798-807

Abstract

Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to this exclusive group, namely, antigen 43 (Ag43), a self-recognizing autotransporter protein. By mass spectrometry Ag43 was demonstrated to be glycosylated by addition of heptose residues at several positions in the passenger domain. Glycosylation of Ag43 by the action of the Aah and TibC glycosyltransferases was observed in laboratory strains. Importantly, Ag43 was also found to be glycosylated in a wild-type strain, suggesting that Ag43-glycosylation may be a widespread phenomenon. Glycosylation of Ag43 does not seem to interfere with its self-associating properties. However, the glycosylated form of Ag43 enhances bacterial binding to human cell lines, whereas the nonglycosylated version of Ag43 does not to confer this property.

Links

PubMed PMC1426561 Online version:10.1128/JB.188.5.1798-1807.2006

Keywords

Adhesins, Bacterial/genetics; Adhesins, Bacterial/metabolism; Amino Acid Sequence; Antigens, Bacterial/genetics; Antigens, Bacterial/metabolism; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Catalytic Domain/genetics; Catalytic Domain/physiology; Cell Line; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Glycosylation; Humans; Molecular Sequence Data

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:AG43

GO:0006486: protein glycosylation

ECO:0000314:

P

Figure 1, Figure 4B

complete
CACAO 6180

ECOLX:F8SKF2

GO:0016757: transferase activity, transferring glycosyl groups

ECO:0000314:

F

Fig 1

complete
CACAO 6182

ECOLX:F8SKF2

enables

GO:0016757: transferase activity, transferring glycosyl groups

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOH1:TIBC

GO:0016757: transferase activity, transferring glycosyl groups

ECO:0000314:

F

Fig 1

complete
CACAO 6184

ECOH1:TIBC

enables

GO:0016757: transferase activity, transferring glycosyl groups

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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