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PMID:16478791
Citation |
Park, KS, Jeon, SH, Kim, SE, Bahk, YY, Holmen, SL, Williams, BO, Chung, KC, Surh, YJ and Choi, KY (2006) APC inhibits ERK pathway activation and cellular proliferation induced by RAS. J. Cell. Sci. 119:819-27 |
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Abstract |
Inactivating mutations in the adenomatous polyposis coli gene (APC), and activating mutations in RAS, occur in a majority of colorectal carcinomas. However, the relationship between these changes and tumorigenesis is poorly understood. RAS-induced activation of the ERK pathway was reduced by overexpressing APC in DLD-1 colorectal cancer cells. ERK activity was increased by Cre-virus-induced Apc knockout in primary Apc(flox/flox) mouse embryonic fibroblasts, indicating that APC inhibits ERK activity. ERK activity was increased by overexpression and decreased by knock down of beta-catenin. The activation of Raf1, MEK and ERK kinases by beta-catenin was reduced by co-expression of APC. These results indicate that APC inhibits the ERK pathway by an action on beta-catenin. RAS-induced activation of the ERK pathway was reduced by the dominant negative form of TCF4, indicating that the ERK pathway regulation by APC/beta-catenin signaling is, at least, partly caused by effects on beta-catenin/TCF4-mediated gene expression. The GTP loading and the protein level of mutated RAS were decreased in cells with reduced ERK activity as a result of APC overexpression, indicating that APC regulates RAS-induced ERK activation at least partly by reduction of the RAS protein level. APC regulates cellular proliferation and transformation induced by activation of both RAS and beta-catenin signaling. |
Links |
PubMed Online version:10.1242/jcs.02779 |
Keywords |
Adenomatous Polyposis Coli Protein/physiology; Animals; Cell Line, Tumor; Cell Proliferation; Extracellular Signal-Regulated MAP Kinases/metabolism; Gene Expression Regulation; Humans; MAP Kinase Signaling System/genetics; MAP Kinase Signaling System/physiology; Mice; NIH 3T3 Cells; Signal Transduction/physiology; TCF Transcription Factors/genetics; TCF Transcription Factors/metabolism; Transcription Factor 7-Like 2 Protein; Transcription Factor AP-1/physiology; beta Catenin/genetics; beta Catenin/metabolism; ets-Domain Protein Elk-1/physiology; ras Proteins/physiology |
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Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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