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PMID:16466397
| Citation |
Birger, Y, Davis, J, Furusawa, T, Rand, E, Piatigorsky, J and Bustin, M (2006) A role for chromosomal protein HMGN1 in corneal maturation. Differentiation 74:19-29 |
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| Abstract |
Corneal differentiation and maturation are associated with major changes in the expression levels of numerous genes, including those coding for the chromatin-binding high-mobility group (HMG) proteins. Here we report that HMGN1, a nucleosome-binding protein that alters the structure and activity of chromatin, affects the development of the corneal epithelium in mice. The corneal epithelium of Hmgn1(-/-) mice is thin, has a reduced number of cells, is poorly stratified, is depleted of suprabasal wing cells, and its most superficial cell layer blisters. In mature Hmgn1(-/-)mice, the basal cells retain the ovoid shape of immature cells, and rest directly on the basal membrane which is disorganized. Gene expression was modified in Hmgn1(-/-) corneas: glutathione-S-transferase (GST)alpha 4 and GST omega 1, epithelial layer-specific markers, were selectively reduced while E-cadherin and alpha-, beta-, and gamma-catenin, components of adherens junctions, were increased. Immunofluorescence analysis reveals a complete co-localization of HMGN1 and p 63 in small clusters of basal corneal epithelial cells of wild-type mice, and an absence of p 63 expressing cells in the central region of the Hmgn1(-/-) cornea. We suggest that interaction of HMGN1 with chromatin modulates the fidelity of gene expression and affects corneal development and maturation. |
| Links |
PubMed Online version:10.1111/j.1432-0436.2006.00054.x |
| Keywords |
Animals; Cell Differentiation; Cells, Cultured; Chromatin/metabolism; Embryo, Mammalian/cytology; Embryo, Mammalian/metabolism; Epithelium, Corneal/cytology; Epithelium, Corneal/metabolism; Fluorescent Antibody Technique; Gene Expression; Gene Expression Regulation, Developmental; HMGN1 Protein/genetics; HMGN1 Protein/physiology; Mice; Mice, Knockout; Phosphoproteins/metabolism; Trans-Activators/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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