PMID:16442857

Citation	Domenichini, S, Raynaud, C, Ni, DA, Henry, Y and Bergounioux, C (2006) Atmnd1-delta1 is sensitive to gamma-irradiation and defective in meiotic DNA repair. DNA Repair (Amst.) 5:455-64
Abstract	The efficient repair of double-strand breaks (DSBs) in genomic DNA is crucial for the survival of all organisms. Mnd1 is suggested to promote the strand invasion step during meiotic recombination. We used a forward genetics approach, through the search for mutants, to characterize the Arabidopsis homologue of Mnd1. Atmnd1 null mutants exhibit normal vegetative and flower development. In contrast, during prophase I, chromosomes become fragmented resulting in random distribution of the fragments between polyads. Male and female meiosis are defective and strong accumulation of AtRAD51 was observed in atmnd1-delta1 nuclei. These results suggest that similarly to its yeast and animal homologues, AtMnd1 plays a role in DSB repair during meiosis. In addition, like its human homologue AtMnd1 is expressed in somatic cells. AtMnd1 expression is strongly induced by gamma-rays and null mutants show increased sensibility to gamma-rays. Taken together, these results suggest that AtMnd1 is also required for DSB repair in somatic cells.
Links	PubMed Online version:10.1016/j.dnarep.2005.12.007
Keywords	Arabidopsis/radiation effects; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Nucleus/metabolism; DNA Repair; DNA, Bacterial/genetics; DNA, Plant/genetics; Exons/genetics; Flowers/cytology; Gamma Rays; Gene Expression Regulation, Plant; Introns/genetics; Meiosis; Mutagenesis, Insertional/genetics; Phenotype; Phylogeny; Plant Infertility; RNA, Messenger/genetics; RNA, Messenger/metabolism; Rad51 Recombinase/metabolism; Radiation Tolerance; Sequence Analysis, DNA
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.