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PMID:16410523
| Citation |
Meyer, P, Saez, L and Young, MW (2006) PER-TIM interactions in living Drosophila cells: an interval timer for the circadian clock. Science 311:226-9 |
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| Abstract |
In contrast to current models, fluorescence resonance energy transfer measurements using a single-cell imaging assay with fluorescent forms of PER and TIM showed that these proteins bind rapidly and persist in the cytoplasm while gradually accumulating in discrete foci. After approximately 6 hours, complexes abruptly dissociated, as PER and TIM independently moved to the nucleus in a narrow time frame. The per(L) mutation delayed nuclear accumulation in vivo and in our cultured cell system, but without affecting rates of PER/TIM assembly or dissociation. This finding points to a previously unrecognized form of temporal regulation that underlies the periodicity of the circadian clock. |
| Links |
PubMed Online version:10.1126/science.1118126 |
| Keywords |
Active Transport, Cell Nucleus; Animals; Cell Line; Cell Nucleus/metabolism; Circadian Rhythm/physiology; Cytoplasm/metabolism; Drosophila Proteins/metabolism; Drosophila melanogaster; Fluorescence Resonance Energy Transfer; Models, Biological; Nuclear Proteins/metabolism; Period Circadian Proteins; Protein Binding; Recombinant Fusion Proteins/metabolism; Time Factors |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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