PMID:16378748

Citation	Lv, B, Shi, T, Wang, X, Song, Q, Zhang, Y, Shen, Y, Ma, D and Lou, Y (2006) Overexpression of the novel human gene, nuclear apoptosis-inducing factor 1, induces apoptosis. Int. J. Biochem. Cell Biol. 38:671-83
Abstract	Apoptosis is a genetically determined cell suicidal program that plays critical roles in many physiological and pathological processes. In this study, we report the cloning and characterization of a novel human gene, nuclear apoptosis-inducing factor 1 (NAIF1), overexpression of which induces apoptosis in cells. Human NAIF1 is located on chromosome 9q34.11 and encodes 327 amino acids with a homeodomain-like region and two nuclear localization signals at its N-terminal region. NAIF1 is conserved across diverse species, including human, mouse, crab-eating macaque, dog, chicken and frog, and shares no obvious homology to any known genes or proteins. Northern blot analysis revealed wide expression of NAIF1 mRNA throughout human tissues. NAIF1 was predominantly localized in the nucleus. Overexpression of NAIF1 inhibited cell growth and induced apoptosis. Furthermore, NAIF1 transfection caused both decreases in mitochondrial membrane potential and caspase-3 activation. In summary, NAIF1 is a nuclear protein that induces apoptosis when overexpressed.
Links	PubMed Online version:10.1016/j.biocel.2005.11.007
Keywords	Active Transport, Cell Nucleus/physiology; Animals; Apoptosis/genetics; Apoptosis Regulatory Proteins/biosynthesis; Apoptosis Regulatory Proteins/genetics; Cell Nucleus/genetics; Cell Nucleus/metabolism; Chromosomes, Human, Pair 9/genetics; Chromosomes, Human, Pair 9/metabolism; Gene Expression; HeLa Cells; Humans; Nuclear Localization Signals/genetics; Nuclear Localization Signals/metabolism; Nuclear Proteins/biosynthesis; Nuclear Proteins/genetics; Transfection
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.