PMID:16344550

Citation	Yang, CK, Kim, JH, Li, H and Stallcup, MR (2006) Differential use of functional domains by coiled-coil coactivator in its synergistic coactivator function with beta-catenin or GRIP1. J. Biol. Chem. 281:3389-97
Abstract	beta-Catenin, a pivotal component of the Wnt-signaling pathway, binds to and serves as a transcriptional coactivator for the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcriptional activator proteins and for the androgen receptor (AR), a nuclear receptor. Three components of the p160 nuclear receptor coactivator complex, including CARM1, p300/CBP, and GRIP1 (one of the p160 coactivators), bind to and cooperate with beta-catenin to enhance transcriptional activation by TCF/LEF and AR. Here we report that another component of the p160 nuclear receptor coactivator complex, the coiled-coil coactivator (CoCoA), directly binds to and cooperates synergistically with beta-catenin as a coactivator for AR and TCF/LEF. CoCoA uses different domains to bind GRIP1 and beta-catenin, and it uses different domains to transmit the activating signal to the transcription machinery, depending on whether it is bound to GRIP1 or beta-catenin. CoCoA associated specifically with the promoters of transiently transfected and endogenous target genes of TCF/LEF, and reduction of the endogenous CoCoA level decreased the ability of TCF/LEF and beta-catenin to activate transcription of transient and endogenous target genes. Thus, CoCoA uses different combinations of functional domains to serve as a physiologically relevant component of the Wnt/beta-catenin signaling pathway and the androgen signaling pathway.
Links	PubMed PMC1626527 Online version:10.1074/jbc.M510403200
Keywords	Animals; COS Cells; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Cell Line; Cell Line, Tumor; Cercopithecus aethiops; Chickens; Chromatin Immunoprecipitation; Enhancer Elements, Genetic; Glutathione Transferase/metabolism; Humans; Immunoprecipitation; Lymphoid Enhancer-Binding Factor 1/metabolism; Models, Biological; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/metabolism; Plasmids/metabolism; Promoter Regions, Genetic; Protein Binding; Protein Interaction Mapping; Protein Structure, Tertiary; RNA Interference; Signal Transduction; TCF Transcription Factors/metabolism; Transcription, Genetic; Transcriptional Activation; Transfection; beta Catenin/metabolism; beta Catenin/physiology
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