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PMID:16339076
| Citation |
Chen, Q, Li, H and De Lozanne, A (2006) Contractile ring-independent localization of DdINCENP, a protein important for spindle stability and cytokinesis. Mol. Biol. Cell 17:779-88 |
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| Abstract |
Dictyostelium DdINCENP is a chromosomal passenger protein associated with centromeres, the spindle midzone, and poles during mitosis and the cleavage furrow during cytokinesis. Disruption of the single DdINCENP gene revealed important roles for this protein in mitosis and cytokinesis. DdINCENP null cells lack a robust spindle midzone and are hypersensitive to microtubule-depolymerizing drugs, suggesting that their spindles may not be stable. Furthermore DdCP224, a protein homologous to the microtubule-stabilizing protein TOGp/XMAP215, was absent from the spindle midzone of DdINCENP null cells. Overexpression of DdCP224 rescued the weak spindle midzone defect of DdINCENP null cells. Although not required for the localization of the myosin II contractile ring and subsequent formation of a cleavage furrow, DdINCENP is important for the abscission of daughter cells at the end of cytokinesis. Finally, we show that the localization of DdINCENP at the cleavage furrow is modulated by myosin II but it occurs by a mechanism different from that controlling the formation of the contractile ring. |
| Links |
PubMed PMC1356588 Online version:10.1091/mbc.E05-08-0704 |
| Keywords |
Animals; Cell Line; Chromosomal Proteins, Non-Histone/analysis; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/physiology; Cloning, Molecular; Cytokinesis/physiology; Dictyostelium/cytology; Dictyostelium/genetics; Dictyostelium/metabolism; Microtubule-Associated Proteins/analysis; Mitosis/genetics; Mitosis/physiology; Mitotic Spindle Apparatus/chemistry; Mitotic Spindle Apparatus/metabolism; Myosin Type II/metabolism; Protozoan Proteins/analysis; Protozoan Proteins/genetics; Protozoan Proteins/physiology; Recombinant Fusion Proteins/analysis; Sequence Homology, Amino Acid |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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