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PMID:16327780
| Citation |
Pinsky, BA, Kung, C, Shokat, KM and Biggins, S (2006) The Ipl1-Aurora protein kinase activates the spindle checkpoint by creating unattached kinetochores. Nat. Cell Biol. 8:78-83 |
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| Abstract |
The spindle checkpoint ensures accurate chromosome segregation by delaying cell-cycle progression until all sister kinetochores capture microtubules from opposite poles and come under tension (for reviews, see refs 1, 2). Although the checkpoint is activated by either the lack of kinetochore-microtubule attachments or defects in the tension exerted by microtubule-generated forces, it is not clear whether these signals are linked. We investigated the connection between tension and attachment by studying the conserved budding yeast Ipl1Aurora protein kinase that is required for checkpoint activation in the absence of tension but not attachment. Here, we show that spindle-checkpoint activation in kinetochore mutants that seem to have unattached kinetochores depends on Ipl1 activity. When Ipl1 function was impaired in these kinetochore mutants, the attachments were restored and the checkpoint was turned off. These data indicate that Ipl1 activates the checkpoint in response to tension defects by creating unattached kinetochores. Moreover, although the Dam1 kinetochore complex has been implicated as a key downstream target, we found the existence of unidentified Ipl1 sites on Dam1 or additional important substrates that regulate both microtuble detachment and the checkpoint. |
| Links |
PubMed Online version:10.1038/ncb1341 |
| Keywords |
Cell Cycle/drug effects; Hot Temperature; Intracellular Signaling Peptides and Proteins; Kinetochores/drug effects; Kinetochores/metabolism; Mitotic Spindle Apparatus/drug effects; Mitotic Spindle Apparatus/metabolism; Mitotic Spindle Apparatus/physiology; Models, Biological; Mutation; Protein Kinases/chemistry; Protein Kinases/physiology; Protein-Serine-Threonine Kinases; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/physiology |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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