PMID:16297388

Citation	Schwarz, M, Knauer, R and Lehle, L (2005) Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit. FEBS Lett. 579:6564-8
Abstract	The key step of N-glycosylation of proteins in the endoplasmic reticulum is catalyzed by the hetero-oligomeric protein complex oligosaccharyltransferase (OST). It transfers the lipid-linked core-oligosaccharide to selected Asn-X-Ser/Thr-sequences of nascent polypeptide chains. Biochemical and genetic approaches have revealed that OST from Saccharomyces cerevisiae consists of nine subunits: Wbp1p, Swp1p, Stt3p, Ost1p, Ost2p, Ost4p, Ost5p, Ostp3 and Ost6p. By blue native polyacrylamide electrophoresis we show that yeast OST consists of two isoforms with distinct functions differing only in the presence of the two related Ost3 and Ost6p proteins. The OST6-complex was found to be important for cell wall integrity and temperature stress. Ost3p and Ost6p are not essential for OST activity, and can in part displace each other in the complex when overexpressed, suggesting a dynamic regulation of the complex formation.
Links	PubMed Online version:10.1016/j.febslet.2005.10.063
Keywords	Cell Wall; Electrophoresis, Polyacrylamide Gel; Hexosyltransferases/chemistry; Hexosyltransferases/physiology; Isoenzymes; Membrane Proteins/chemistry; Membrane Proteins/physiology; Multiprotein Complexes/chemistry; Multiprotein Complexes/physiology; Protein Subunits; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/physiology
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