PMID:16245339

Citation	Lancman, JJ, Caruccio, NC, Harfe, BD, Pasquinelli, AE, Schageman, JJ, Pertsemlidis, A and Fallon, JF (2005) Analysis of the regulation of lin-41 during chick and mouse limb development. Dev. Dyn. 234:948-60
Abstract	We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin-41. During limb development, lin-41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin-41 activity is temporally regulated by miRNAs (let-7 and lin-4) that bind to complementary sites in the lin-41 3'-untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3'-UTR of chicken lin-41, including sites for the chicken orthologues of both C. elegans let-7 and lin-4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin-41 activity in vivo.
Links	PubMed Online version:10.1002/dvdy.20591
Keywords	Amino Acid Sequence; Animals; Base Sequence; Binding Sites/genetics; Blotting, Northern; Chick Embryo; Cloning, Molecular; Computational Biology; Extremities/embryology; Gene Components; Gene Expression Regulation, Developmental; In Situ Hybridization; Mice; Mice, Mutant Strains; MicroRNAs/genetics; MicroRNAs/metabolism; Microspheres; Molecular Sequence Data; Sequence Alignment; Sequence Analysis, DNA; Signal Transduction/genetics; Species Specificity; Transcription Factors/genetics; Transcription Factors/metabolism
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