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PMID:16227588

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Citation

Takahashi, Y, Karbowski, M, Yamaguchi, H, Kazi, A, Wu, J, Sebti, SM, Youle, RJ and Wang, HG (2005) Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol. Cell. Biol. 25:9369-82

Abstract

Bif-1, a member of the endophilin B protein family, interacts with Bax and promotes interleukin-3 withdrawal-induced Bax conformational change and apoptosis when overexpressed in FL5.12 cells. Here, we provide evidence that Bif-1 plays a regulatory role in apoptotic activation of not only Bax but also Bak and appears to be involved in suppression of tumorigenesis. Inhibition of endogenous Bif-1 expression in HeLa cells by RNA interference abrogated the conformational change of Bax and Bak, cytochrome c release, and caspase 3 activation induced by various intrinsic death signals. Similar results were obtained in Bif-1 knockout mouse embryonic fibroblasts. While Bif-1 did not directly interact with Bak, it heterodimerized with Bax on mitochondria in intact cells, and this interaction was enhanced by apoptosis induction and preceded the Bax conformational change. Moreover, suppression of Bif-1 expression was associated with an enhanced ability of HeLa cells to form colonies in soft agar and tumors in nude mice. Taken together, these findings support the notion that Bif-1 is an important component of the mitochondrial pathway for apoptosis as a novel Bax/Bak activator, and loss of this proapoptotic molecule may contribute to tumorigenesis.

Links

PubMed PMC1265816 Online version:10.1128/MCB.25.21.9369-9382.2005

Keywords

Adaptor Proteins, Signal Transducing/genetics; Adaptor Proteins, Signal Transducing/metabolism; Animals; Apoptosis/physiology; Caspases/metabolism; Cells, Cultured; Cytochromes c/metabolism; Enzyme Activation; Fibroblasts/physiology; HeLa Cells; Humans; Mice; Mice, Knockout; Mitochondria/physiology; Neoplasms, Experimental/genetics; Protein Conformation; RNA, Small Interfering/metabolism; bcl-2 Homologous Antagonist-Killer Protein/metabolism; bcl-2-Associated X Protein/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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