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PMID:16219773
| Citation |
Tulin, A, Naumova, NM, Menon, AK and Spradling, AC (2006) Drosophila poly(ADP-ribose) glycohydrolase mediates chromatin structure and SIR2-dependent silencing. Genetics 172:363-71 |
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| Abstract |
Protein ADP ribosylation catalyzed by cellular poly(ADP-ribose) polymerases (PARPs) and tankyrases modulates chromatin structure, telomere elongation, DNA repair, and the transcription of genes involved in stress resistance, hormone responses, and immunity. Using Drosophila genetic tools, we characterize the expression and function of poly(ADP-ribose) glycohydrolase (PARG), the primary enzyme responsible for degrading protein-bound ADP-ribose moieties. Strongly increasing or decreasing PARG levels mimics the effects of Parp mutation, supporting PARG's postulated roles in vivo both in removing ADP-ribose adducts and in facilitating multiple activity cycles by individual PARP molecules. PARP is largely absent from euchromatin in PARG mutants, but accumulates in large nuclear bodies that may be involved in protein recycling. Reducing the level of either PARG or the silencing protein SIR2 weakens copia transcriptional repression. In the absence of PARG, SIR2 is mislocalized and hypermodified. We propose that PARP and PARG promote chromatin silencing at least in part by regulating the localization and function of SIR2 and possibly other nuclear proteins. |
| Links |
PubMed PMC1456164 Online version:10.1534/genetics.105.049239 |
| Keywords |
Adenosine Diphosphate Ribose/metabolism; Animals; Cell Nucleus/metabolism; Chromatin/genetics; Chromatin/metabolism; DNA Transposable Elements/genetics; Drosophila Proteins/antagonists & inhibitors; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Drosophila melanogaster/metabolism; Female; Gene Silencing; Glycoside Hydrolases/genetics; Glycoside Hydrolases/metabolism; Histone Deacetylase Inhibitors; Histone Deacetylases/genetics; Histone Deacetylases/metabolism; Male; Mutation; Peptide Hydrolases/genetics; Peptide Hydrolases/metabolism; Poly(ADP-ribose) Polymerases/genetics; Poly(ADP-ribose) Polymerases/metabolism; Retroelements; Sirtuins/antagonists & inhibitors; Sirtuins/genetics; Sirtuins/metabolism |
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Significance
Annotations
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