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PMID:16216585
| Citation |
Zhang, X, Ji, Y, Zhang, L, Harrison, SC, Marinescu, DC, Nibert, ML and Baker, TS (2005) Features of reovirus outer capsid protein mu1 revealed by electron cryomicroscopy and image reconstruction of the virion at 7.0 Angstrom resolution. Structure 13:1545-57 |
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| Abstract |
Reovirus is a useful model for addressing the molecular basis of membrane penetration by one of the larger nonenveloped animal viruses. We now report the structure of the reovirus virion at approximately 7.0 A resolution as obtained by electron cryomicroscopy and three-dimensional image reconstruction. Several features of the myristoylated outer capsid protein mu1, not seen in a previous X-ray crystal structure of the mu1-sigma3 heterohexamer, are evident in the virion. These features appear to be important for stabilizing the outer capsid, regulating the conformational changes in mu1 that accompany perforation of target membranes, and contributing directly to membrane penetration during cell entry. |
| Links |
PubMed Online version:10.1016/j.str.2005.07.012 |
| Keywords |
Algorithms; Amino Acid Sequence; Animals; Capsid Proteins/chemistry; Capsid Proteins/isolation & purification; Capsid Proteins/ultrastructure; Cryoelectron Microscopy; Crystallography, X-Ray; Fourier Analysis; L Cells (Cell Line); Mice; Models, Chemical; Models, Molecular; Molecular Sequence Data; Myristic Acid/metabolism; Orthoreovirus/chemistry; Orthoreovirus/genetics; Orthoreovirus/ultrastructure; Protein Conformation; Protein Structure, Secondary; Sequence Homology, Amino Acid; Virion/growth & development; Virion/ultrastructure |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019028: viral capsid |
ECO:0000314: |
C |
Electron Cryomicroscopy of viral capsid shown in Figure 2 |
complete | ||||
|
part_of |
GO:0019028: viral capsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019028: viral capsid |
ECO:0000269: |
C |
Electron Cryomicroscopy data of viral capsid in Figure 2 |
complete | ||||
|
part_of |
GO:0019028: viral capsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019028: viral capsid |
ECO:0000314: |
C |
Electron Cryomicroscopy data of viral capsid in Figure 2 |
complete | ||||
|
part_of |
GO:0019030: icosahedral viral capsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019030: icosahedral viral capsid |
ECO:0000314: |
C |
Electron Cryomicroscopy data of viral capsid in Figure 2 |
complete | ||||
See also
References
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