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PMID:16204217

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Citation

Merritt, TJ, Sezgin, E, Zhu, CT and Eanes, WF (2006) Triglyceride pools, flight and activity variation at the Gpdh locus in Drosophila melanogaster. Genetics 172:293-304

Abstract

We have created a set of P-element excision-derived Gpdh alleles that generate a range of GPDH activity phenotypes ranging from zero to full activity. By placing these synthetic alleles in isogenic backgrounds, we characterize the effects of minor and major activity variation on two different aspects of Gpdh function: the standing triglyceride pool and glycerol-3-phosphate shuttle-assisted flight. We observe small but statistically significant reductions in triglyceride content for adult Gpdh genotypes possessing 33-80% reductions from normal activity. These small differences scale to a notable proportion of the observed genetic variation in triglyceride content in natural populations. Using a tethered fly assay to assess flight metabolism, we observed that genotypes with 100 and 66% activity exhibited no significant difference in wing-beat frequency (WBF), while activity reductions from 60 to 10% showed statistically significant reductions of approximately 7% in WBF. These studies show that the molecular polymorphism associated with GPDH activity could be maintained in natural populations by selection in the triglyceride pool.

Links

PubMed PMC1456158 Online version:10.1534/genetics.105.047035

Keywords

Animals; Cell Survival/drug effects; Drosophila melanogaster/enzymology; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Energy Metabolism; Female; Flight, Animal; Gene Expression Regulation, Developmental; Genetic Variation; Genotype; Glycerolphosphate Dehydrogenase/genetics; Glycerophosphates/metabolism; Male; Phenotype; Polymorphism, Genetic; Selection, Genetic; Triglycerides/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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