PMID:16199027

Citation	Hyenne, V, Louvet-Vallée, S, El-Amraoui, A, Petit, C, Maro, B and Simmler, MC (2005) Vezatin, a protein associated to adherens junctions, is required for mouse blastocyst morphogenesis. Dev. Biol. 287:180-91
Abstract	Cell-cell interactions play a major role during preimplantation development of the mouse embryo. The formation of adherens junctions is a major feature of compaction, the first morphogenetic event that takes place at the 8-cell stage. Then, during the following two cell cycles, tight junctions form, and the outer layer of cells differentiate into a functional epithelium, leading to the formation of the blastocoel cavity. Until now, E-cadherin was the only transmembrane molecule localized in adherens junctions and required for early development. Vezatin is a transmembrane protein of adherens junctions, interacting with the E-cadherin-catenins complex. Here, we show that vezatin is expressed very early during mouse preimplantation development. It co-localizes with E-cadherin throughout development, being found all around the cell cortex before compaction and basolaterally in adherens junctions thereafter. In addition, vezatin is also detected in nuclei during most of the cell cycle. Finally, using a morpholino-oligonucleotide approach to inhibit vezatin function during preimplantation development, we observed that inhibition of vezatin synthesis leads to a cell cycle arrest with limited cell-cell interactions. This phenotype can be rescued when mRNAs coding for vezatin missing the 5'UTR are co-injected with the anti-vezatin morpholino-oligonucleotide. Cells derived from blastomeres injected with morpholino-oligonucleotide had a reduced amount of vezatin concomitantly with a decrease in the quantity of E-cadherin and beta-catenin localized in the areas of intercellular contact. Shift in E-cadherin cortical distribution was correlated with a strong decrease in E-cadherin mRNA and protein contents. Altogether, these observations demonstrate that vezatin is required for morphogenesis of the preimplantation mouse embryo.
Links	PubMed Online version:10.1016/j.ydbio.2005.09.004
Keywords	Adherens Junctions/physiology; Animals; Blastocyst/physiology; Cadherins/genetics; Cadherins/metabolism; Carrier Proteins/biosynthesis; Carrier Proteins/genetics; Carrier Proteins/physiology; Cell Membrane/metabolism; Cell Nucleus/metabolism; Female; Male; Membrane Proteins/biosynthesis; Membrane Proteins/genetics; Membrane Proteins/physiology; Mice; Molecular Sequence Data; Oligonucleotides; Protein Isoforms/genetics; Protein Isoforms/metabolism; RNA, Messenger/metabolism
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