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PMID:16168084
| Citation |
Eide, DJ, Clark, S, Nair, TM, Gehl, M, Gribskov, M, Guerinot, ML and Harper, JF (2005) Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae. Genome Biol. 6:R77 |
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| Abstract |
Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'. |
| Links |
PubMed PMC1242212 Online version:10.1186/gb-2005-6-9-r77 |
| Keywords |
Elements; Gene Deletion; Gene Expression Profiling; Genes, Fungal/genetics; Genome, Fungal/genetics; Homeostasis/physiology; Minerals/metabolism; Mitochondria/metabolism; Organelles/metabolism; Phenotype; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Spectrophotometry, Atomic; Trace Elements/metabolism; Vacuoles/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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