PMID:16159778

Citation	Real, G, Pinto, SM, Schyns, G, Costa, T, Henriques, AO and Moran, CP Jr (2005) A gene encoding a holin-like protein involved in spore morphogenesis and spore germination in Bacillus subtilis. J. Bacteriol. 187:6443-53
Abstract	We report here studies of expression and functional analysis of a Bacillus subtilis gene, ywcE, which codes for a product with features of a holin. Primer extension analysis of ywcE transcription revealed that a single transcript accumulated from the onset of sporulation onwards, produced from a sigma(A)-type promoter bearing the TG dinucleotide motif of "extended" -10 promoters. No primer extension product was detected in vivo during growth. However, specific runoff products were produced in vitro from the ywcE promoter by purified sigma(A)-containing RNA polymerase (Esigma(A)), and the in vivo and in vitro transcription start sites were identical. These results suggested that utilization of the ywcE promoter by Esigma(A) during growth was subjected to repression. Studies with a lacZ fusion revealed that the transition-state regulator AbrB repressed the transcription of ywcE during growth. This repression was reversed at the onset of sporulation in a Spo0A-dependent manner, but Spo0A did not appear to contribute otherwise to ywcE transcription. We found ywcE to be required for proper spore morphogenesis. Spores of the ywcE mutant showed a reduced outer coat which lacked the characteristic striated pattern, and the outer coat failed to attach to the underlying inner coat. The mutant spores also accumulated reduced levels of dipicolinic acid. ywcE was also found to be important for spore germination.
Links	PubMed PMC1236627 Online version:10.1128/JB.187.18.6443-6453.2005
Keywords	Bacillus subtilis/genetics; Bacillus subtilis/physiology; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Morphogenesis; Spores, Bacterial/growth & development; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:YWCE		GO:0009847: spore germination	ECO:0000315:		P		Figure 7	complete CACAO 2936
BACSU:YWCE	involved_in	GO:0009847: spore germination	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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