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PMID:16131612
| Citation |
Yoshikawa, M, Peragine, A, Park, MY and Poethig, RS (2005) A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis. Genes Dev. 19:2164-75 |
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| Abstract |
The Arabidopsis genes, TAS2 and TAS1a, produce structurally similar noncoding transcripts that are transformed into short (21-nucleotide [nt]) and long (24-nt) siRNAs by RNA silencing pathways. Some of these short siRNAs direct the cleavage of protein-coding transcripts, and thus function as trans-acting siRNAs (ta-siRNAs). Using genetic analysis, we defined the pathway by which ta-siRNAs and other short siRNAs are generated from these loci. This process is initiated by the miR173-directed cleavage of a primary poly(A) transcript. The 3' fragment is then transformed into short siRNAs by the sequential activity of SGS3, RDR6, and DCL4: SGS3 stabilizes the fragment, RDR6 produces a complementary strand, and DCL4 cleaves the resulting double-stranded molecule into short siRNAs, starting at the end with the miR173 cleavage site and proceeding in 21-nt increments from this point. The 5' cleavage fragment is also processed by this pathway, but less efficiently. The DCL3-dependent pathway that generates long siRNAs does not require miRNA-directed cleavage and plays a minor role in the silencing of these loci. Our results define the core components of a post-transcriptional gene silencing pathway in Arabidopsis and reveal some of the features that direct transcripts to this pathway. |
| Links |
PubMed PMC1221887 Online version:10.1101/gad.1352605 |
| Keywords |
Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Gene Expression Regulation, Plant; Gene Targeting; Genes, Plant; MicroRNAs/metabolism; Models, Biological; Molecular Sequence Data; Mutation; Protein Structure, Tertiary; RNA Interference; RNA Processing, Post-Transcriptional; RNA, Small Interfering/biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic |
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Significance
Annotations
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