GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16096640
| Citation |
Pardo, B and Marcand, S (2005) Rap1 prevents telomere fusions by nonhomologous end joining. EMBO J. 24:3117-27 |
|---|---|
| Abstract |
Telomeres protect chromosomes from end-to-end fusions. In yeast Saccharomyces cerevisiae, the protein Rap1 directly binds telomeric DNA. Here, we use a new conditional allele of RAP1 and show that Rap1 loss results in frequent fusions between telomeres. Analysis of the fusion point with restriction enzymes indicates that fusions occur between telomeres of near wild-type length. Telomere fusions are not observed in cells lacking factors required for nonhomologous end joining (NHEJ), including Lig4 (ligase IV), KU and the Mre11 complex. SAE2 and TEL1 do not affect the frequency of fusions. Together, these results show that Rap1 is essential to block NHEJ between telomeres. Since the presence of Rap1 at telomeres has been conserved through evolution, the establishment of NHEJ suppression by Rap1 could be universal. |
| Links |
PubMed PMC1201357 Online version:10.1038/sj.emboj.7600778 |
| Keywords |
Cell Survival/physiology; Chromosomes, Fungal/genetics; DNA Ligases/metabolism; DNA Repair; DNA Restriction Enzymes/chemistry; Endodeoxyribonucleases/metabolism; Endonucleases; Exodeoxyribonucleases/metabolism; Fungal Proteins/metabolism; Intracellular Signaling Peptides and Proteins; Protein-Serine-Threonine Kinases; Recombination, Genetic; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Telomere/genetics; Telomere/physiology; Telomere-Binding Proteins/chemistry; Telomere-Binding Proteins/genetics; Telomere-Binding Proteins/physiology; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
