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PMID:16086103
| Citation |
Wu, LT, Tsou, MF, Ho, CC, Chuang, JY, Kuo, HM and Chung, JG (2005) Berberine inhibits arylamine N-acetyltransferase activity and gene expression in Salmonella typhi. Curr. Microbiol. 51:255-61 |
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| Abstract |
The effects of berberine on growth, arylamine N-acetyltransferase (NAT) activity, and gene expression in Salmonella Typhi (Typhi) were described. The growth inhibition of Typhi was determined by measuring absorbance by optical density (OD at 650 nm). The NAT activity was determined by measuring the levels of 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF) by high-performance liquid chromatography. The results demonstrated that 24-h berberine treatment decreased bacteria growth and amounts of AAF in Typhi. Western blotting and flow cytometry were used for examining the levels of NAT after bacteria were cotreated with or without various concentrations of berberine, and results indicated that berberine decreased the levels of NAT in Typhi. Polymerase chain reaction was used for examining the gene expression of NAT (mRNA NAT), and results indicated that berberine affects mRNA NAT1 expression in Typhi. |
| Links |
PubMed Online version:10.1007/s00284-005-4569-7 |
| Keywords |
Arylamine N-Acetyltransferase/metabolism; Berberine/pharmacology; Berberis; Down-Regulation; Gene Expression Regulation, Bacterial/drug effects; RNA, Messenger/metabolism; Salmonella typhi/drug effects; Salmonella typhi/genetics; Salmonella typhi/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008080: N-acetyltransferase activity |
ECO:0000315: |
F |
Figures 1 and 2 show acetyl transferase activity |
complete | ||||
See also
References
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