GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16083859
| Citation |
Jiang, GJ, Pan, L, Huang, XY, Han, M, Wen, JK and Sun, FZ (2005) Expression of HSG is essential for mouse blastocyst formation. Biochem. Biophys. Res. Commun. 335:351-5 |
|---|---|
| Abstract |
It has been shown recently that hyperplasia suppressor gene (HSG) is a powerful regulator for cell proliferation and has a critical role in mitochondrial fusion in many cells. However, little is known about its expression, localization, and function during oocyte maturation and early embryogenesis. In this study, with indirect immunofluorescent staining and Western blotting, we found that HSG was expressed in mouse oocytes and preimplantation embryos which primarily exhibited a submembrane distribution pattern in the cytoplasm. Moreover, HSG mainly associated with beta-tubulin during oocyte maturation and early embryonic development. When mouse zygotes were injected with HSG antisense plasmid and cultured in vitro, their capacity to form blastocysts was severely impaired. Our results indicate that HSG plays an essential role in mouse preimplantation development. |
| Links |
PubMed Online version:10.1016/j.bbrc.2005.07.088 |
| Keywords |
Animals; Blastocyst/cytology; Blotting, Western; Cell Proliferation; Cloning, Molecular; Cytoplasm/metabolism; Fluorescent Antibody Technique, Indirect; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/physiology; Gene Expression Regulation, Developmental; Humans; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Mitochondria/metabolism; Oligonucleotides, Antisense/chemistry; Oocytes/cytology; Oocytes/metabolism; Plasmids/metabolism; Rats; Tubulin/chemistry; Tubulin/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
