GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16076953
| Citation |
Meister, S, Kanzok, SM, Zheng, XL, Luna, C, Li, TR, Hoa, NT, Clayton, JR, White, KP, Kafatos, FC, Christophides, GK and Zheng, L (2005) Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. Proc. Natl. Acad. Sci. U.S.A. 102:11420-5 |
|---|---|
| Abstract |
We show that, in the malaria vector Anopheles gambiae, expression of Cecropin 1 is regulated by REL2, an NF-kappaB-like transcription factor orthologous to Drosophila Relish. Through alternative splicing, REL2 produces a full-length (REL2-F) and a shorter (REL2-S) protein isoform lacking the inhibitory ankyrin repeats and death domain. RNA interference experiments show that, in contrast to Drosophila Relish, which responds solely to Gram-negative bacteria, the Anopheles REL2-F and REL2-S isoforms are involved in defense against the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli bacteria, respectively. REL2-F also regulates the intensity of mosquito infection with the malaria parasite, Plasmodium berghei. The adaptor IMD shares the same activities as REL2-F. Microarray analysis identified 10 additional genes regulated by REL2, including CEC3, GAM1, and LRIM1. |
| Links |
PubMed PMC1183586 Online version:10.1073/pnas.0504950102 |
| Keywords |
Alternative Splicing/genetics; Animals; Anopheles gambiae/immunology; Anopheles gambiae/microbiology; Anopheles gambiae/parasitology; Escherichia coli/immunology; Gene Expression Regulation/immunology; Genome Components; Insect Proteins/genetics; Insect Proteins/immunology; Insect Proteins/metabolism; Luciferases; Microarray Analysis; Plasmodium berghei/immunology; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction/immunology; Staphylococcus aureus/immunology; Transcription Factors/immunology; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0002218: activation of innate immune response |
ECO:0000315: |
P |
Figure 3A shows that silencing of either domain of REL2 results in significant reduction of CEC1 (Cecropin A) expression. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
