PMID:16054027

Citation	Jékely, G, Sung, HH, Luque, CM and Rørth, P (2005) Regulators of endocytosis maintain localized receptor tyrosine kinase signaling in guided migration. Dev. Cell 9:197-207
Abstract	Guidance receptors detect extracellular cues and instruct migrating cells how to orient in space. Border cells perform a directional invasive migration during Drosophila oogenesis and use two receptor tyrosine kinases (RTKs), EGFR and PVR (PDGF/VEGF Receptor), to read guidance cues. We find that spatial localization of RTK signaling within these migrating cells is actively controlled. Border cells lacking Cbl, an RTK-associated E3 ubiquitin ligase, have delocalized guidance signaling, resulting in severe migration defects. Absence of Sprint, a receptor-recruited, Ras-activated Rab5 guanine exchange factor, gives related defects. In contrast, increasing the level of RTK signaling by receptor overexpression or removing Hrs and thereby decreasing RTK degradation does not perturb migration. Cbl and Sprint both regulate early steps of RTK endocytosis. Thus, a physiological role of RTK endocytosis is to ensure localized intracellular response to guidance cues by stimulating spatial restriction of signaling.
Links	PubMed Online version:10.1016/j.devcel.2005.06.004
Keywords	Animals; Carrier Proteins/genetics; Carrier Proteins/physiology; Cell Movement/physiology; Drosophila; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Endocytosis/physiology; Endosomal Sorting Complexes Required for Transport; Female; Mutation; Oocytes/physiology; Phosphoproteins/genetics; Phosphoproteins/physiology; Protein Kinases/genetics; Protein Kinases/physiology; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/physiology; Proto-Oncogene Proteins c-cbl; Receptor Protein-Tyrosine Kinases/genetics; Receptor Protein-Tyrosine Kinases/physiology; Receptor, Epidermal Growth Factor/genetics; Receptor, Epidermal Growth Factor/physiology; Receptors, Invertebrate Peptide/genetics; Receptors, Invertebrate Peptide/physiology; Receptors, Platelet-Derived Growth Factor/genetics; Receptors, Platelet-Derived Growth Factor/physiology; Receptors, Vascular Endothelial Growth Factor/genetics; Receptors, Vascular Endothelial Growth Factor/physiology; Signal Transduction/physiology; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/physiology
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