PMID:16045620

Citation	Pham, VD, Shebelut, CW, Zumstein, EJ and Singer, M (2005) BrgE is a regulator of Myxococcus xanthus development. Mol. Microbiol. 57:762-73
Abstract	We report here the identification and characterization of a member of the Myxococcus xanthus SdeK signal transduction pathway, BrgE. This protein was identified as an SdeK-interacting component using a yeast two-hybrid screen, and we further confirmed this interaction by the glutathione S-transferase (GST) pulldown assay. Additional yeast two-hybrid analyses revealed that BrgE preferentially interacts with the putative amino-terminal sensor domain of SdeK, but not with the carboxy-terminal kinase domain. A brgE insertion strain was shown to be blocked in development between aggregation and mound formation, and decreased by 50-fold in viable spore production compared with the parental wild type. These phenotypes are similar to those of sdeK mutants. The brgE mutation also altered expression of a sample of Tn5 lac developmental markers that are also SdeK regulated. Finally, we demonstrated that a brgE sdeK double mutant has a more severe sporulation defect than either of the two single mutants, suggesting that BrgE and SdeK act synergistically to regulate wild-type levels of sporulation. In sum, these data suggest that BrgE operates as an auxiliary factor to stimulate the SdeK signal transduction pathway by directly binding to the amino-terminal sensor domain of SdeK.
Links	PubMed Online version:10.1111/j.1365-2958.2005.04726.x
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Culture Media; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutation; Myxococcus xanthus/genetics; Myxococcus xanthus/growth & development; Myxococcus xanthus/metabolism; Myxococcus xanthus/physiology; Signal Transduction; Spores, Bacterial/growth & development; Two-Hybrid System Techniques
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MYXXA:O52237	involved_in	GO:0042594: response to starvation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
MYXXA:O52237		GO:0042594: response to starvation	ECO:0000315:		P		Fig. 4. Developmental progression of wild-type cells and various mutants on TPM starvation agar during a 72 h time-course. Strains used were DK1622 (parent wild type), VP664 (brgE), DK7862 (sdeK), VP1014 (brgE sdeK), DK5208 (csgA) and VP825 (brgE csgA)	complete CACAO 8340
MYXXA:O52237		GO:0005515: protein binding	ECO:0000021:	PMID:16045620	F		Fig 1 GST pulldown assay	complete CACAO 8365

References

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