GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16041126
| Citation |
Shimaoka, M, Kawasaki, H, Takenaka, Y, Kurahashi, O and Matsui, H (2005) Effects of edd and pgi disruptions on inosine accumulation in Escherichia coli. Biosci. Biotechnol. Biochem. 69:1248-55 |
|---|---|
| Abstract |
Using an inosine-producing mutant of Escherichia coli, the contributions of the central carbon metabolism for overproducing inosine were investigated. Sodium gluconate instead of glucose was tested as a carbon source to increase the supply of ribose-5-phosphate through the oxidative pentose phosphate pathway. The edd (6-phosphogluconate dehydrase gene)-disrupted mutant accumulated 2.5 g/l of inosine from 48 g/l of sodium gluconate, compared with 1.4 g/l of inosine in the edd wild strain. The rpe (ribulose phosphate 3-epimerase gene)-disrupted mutant resulted in low cell growth and low inosine production on glucose and on gluconate. The disruption of pgi (glucose-6-phosphate isomerase gene) was effective for increasing the accumulation of inosine from glucose but resulted in low cell growth. The pgi-disrupted mutant accumulated 3.7 g/l of inosine from 40 g/l of glucose when 8 g/l of yeast extract was added to the medium. Furthermore, to improve effective utilization of adenine, the yicP (adenine deaminase gene)-disrupted mutant was evaluated. It showed higher inosine accumulation, of 3.7 g/l, than that of 2.8 g/l in the yicP wild strain when 4 g/l of yeast extract was added to the medium. |
| Links | |
| Keywords |
Adenine/pharmacology; Aminohydrolases/genetics; Aminohydrolases/metabolism; Cell Division/drug effects; Cell Division/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Gluconates/pharmacology; Glucose/pharmacology; Glucose-6-Phosphate Isomerase/genetics; Glucose-6-Phosphate Isomerase/metabolism; Glucosephosphate Dehydrogenase/genetics; Glucosephosphate Dehydrogenase/metabolism; Hydro-Lyases/genetics; Hydro-Lyases/metabolism; Inosine/biosynthesis; Mutation; Time Factors |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
