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PMID:1601874
| Citation |
Hanlon, DW, Márquez-Magaña, LM, Carpenter, PB, Chamberlin, MJ and Ordal, GW (1992) Sequence and characterization of Bacillus subtilis CheW. J. Biol. Chem. 267:12055-60 |
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| Abstract |
A Bacillus subtilis open reading frame (ORF) encoding a predicted polypeptide of 156 amino acids was subcloned and sequenced. The polypeptide was found to be homologous to CheW of Escherichia coli, sharing 28.6% amino acid identity. The ORF was verified by using a bacteriophage T7 expression system in E. coli. The gene was inactivated by insertion of a nonpolor chloramphenicol acetyltransferase cassette in its N-terminal region. In the absence of chemoeffectors, the mutant displayed a smooth swimming bias, with some tumbling. The CheW- mutant was defective on swarm plates but was complemented by a plasmid that expressed wild type CheW. Addition of attractant or repellent to the CheW- mutant resulted in transient smooth swimming or tumbling, respectively. However, capillary assays revealed that chemotaxis was substantially impaired in the mutant strain. |
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| Keywords |
Amino Acid Sequence; Aspartic Acid/metabolism; Bacillus subtilis/genetics; Bacterial Proteins/genetics; Base Sequence; Blotting, Southern; Chemotactic Factors/genetics; Chloramphenicol O-Acetyltransferase/genetics; DNA, Bacterial/genetics; Deoxyglucose/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli Proteins; Genes, Bacterial; Genetic Complementation Test; Molecular Sequence Data; Open Reading Frames; Plasmids; Restriction Mapping; Sequence Alignment |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0042333: chemotaxis to oxidizable substrate |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0042333: chemotaxis to oxidizable substrate |
ECO:0000315: |
P |
Figure 6. Complementation of the CheW null mutant on tryptone (A and B) and mannitol (C and D) swarm plates. |
complete | ||||
Notes
See also
References
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