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PMID:16001968
| Citation |
Sugiura, M, Fuke, S, Suo, S, Sasagawa, N, Van Tol, HH and Ishiura, S (2005) Characterization of a novel D2-like dopamine receptor with a truncated splice variant and a D1-like dopamine receptor unique to invertebrates from Caenorhabditis elegans. J. Neurochem. 94:1146-57 |
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| Abstract |
We have cloned two novel Caenorhabditis elegans dopamine receptors, DOP-3 and DOP-4. DOP-3 shows high sequence homology with other D2-like dopamine receptors. As a result of alternative splicing, a truncated splice variant of DOP-3, DOP-3nf, was produced. Because of the in-frame insertion of a stop codon in the third intracellular loop, DOP-3nf lacks the sixth and seventh transmembrane domains that are found in the full-length DOP-3 receptor. Reporter gene assay showed that DOP-3 attenuates forskolin-stimulated cAMP formation in response to dopamine stimulation, whereas DOP-3nf does not. When DOP-3 was coexpressed with DOP-3nf, the ability to inhibit forskolin-stimulated cAMP formation was reduced. DOP-4 shows high sequence homology with D1-like dopamine receptors unique to invertebrates, which are distinct from mammalian D1-like dopamine receptors. Reporter gene assay showed that DOP-4 stimulates cAMP accumulation in response to dopamine stimulation. These two receptors provide new opportunities to understand dopaminergic signaling at the molecular level. |
| Links |
PubMed Online version:10.1111/j.1471-4159.2005.03268.x |
| Keywords |
Amino Acid Sequence; Animals; CHO Cells; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Cell Line; Cloning, Molecular; Cricetinae; Cricetulus; DNA, Recombinant; Female; Genetic Variation; Humans; Ligands; Male; Molecular Sequence Data; Peptide Fragments/genetics; Peptide Fragments/metabolism; Peptide Fragments/physiology; Receptors, Dopamine D2/genetics; Receptors, Dopamine D2/metabolism; Receptors, Dopamine D2/physiology; Tissue Distribution |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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