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PMID:15994834
| Citation |
Reuven, NB and Weller, SK (2005) Herpes simplex virus type 1 single-strand DNA binding protein ICP8 enhances the nuclease activity of the UL12 alkaline nuclease by increasing its processivity. J. Virol. 79:9356-8 |
|---|---|
| Abstract |
UL12 is a 5'- to 3'-exonuclease encoded by herpes simplex virus type 1 (HSV-1) which degrades single- and double-stranded DNA. UL12 and the single-strand DNA binding protein ICP8 mediate a strand exchange reaction. We found that ICP8 inhibited UL12 digestion of single-stranded DNA but stimulated digestion of double-stranded DNA threefold. The stimulatory effect of ICP8 was independent of a strand exchange reaction; furthermore, the effect was specific to ICP8, as it could not be reproduced by Escherichia coli single-stranded DNA binding protein. The effect of ICP8 on the rate of UL12 double-stranded DNA digestion is attributable to an increase in processivity in the presence of ICP8. |
| Links |
PubMed PMC1168754 Online version:10.1128/JVI.79.14.9356-9358.2005 |
| Keywords |
DNA/metabolism; DNA-Binding Proteins/physiology; Ribonucleases/metabolism; Viral Proteins/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004518: nuclease activity |
ECO:0000314: |
F |
Fig 1. |
complete | ||||
|
enables |
GO:0004518: nuclease activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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