PMID:15982649

Citation	Herrero, MB, Mandal, A, Digilio, LC, Coonrod, SA, Maier, B and Herr, JC (2005) Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization. Dev. Biol. 284:126-42
Abstract	This study demonstrates the retention of mouse sperm lysozyme-like protein (mSLLP1) in the equatorial segment of spermatozoa following the acrosome reaction and a role for mSLLP1 in sperm-egg binding and fertilization. Treatment of cumulus intact oocytes with either recmSLLP1 or its antiserum resulted in a significant (P < or = 0.05) inhibition of fertilization. Co-incubation of zona-free mouse oocytes with capacitated mouse spermatozoa in the presence of varying concentrations of anti-recmSLLP1 serum or recmSLLP1 also inhibited sperm-oolemma binding. A complete inhibition of binding and fusion of spermatozoa to the oocyte occurred at 12.5 muM concentration of recmSLLP1, while conventional chicken and human lysozymes did not block sperm-egg binding. mSLLP1 showed receptor sites in the perivitelline space as well as on the microvillar region of the egg plasma membrane. The retention of mSLLP1 in the equatorial segment of acrosome-reacted sperm, the inhibitory effects of both recmSLLP1 and antibodies to SLLP1 on in vitro fertilization with both cumulus intact and zona-free eggs, and the definition of complementary SLLP1-binding sites on the egg plasma membrane together support the hypothesis that a c lysozyme-like protein is involved in the binding of spermatozoa to the egg plasma membrane during fertilization.
Links	PubMed Online version:10.1016/j.ydbio.2005.05.008
Keywords	Amino Acid Sequence; Animals; Base Sequence; Blotting, Western; Chickens; Cloning, Molecular; DNA Primers; Dose-Response Relationship, Drug; Female; Fertilization/drug effects; Fertilization/genetics; Fertilization/physiology; Fertilization in Vitro; Fluorescent Antibody Technique, Indirect; Humans; Isoantigens/genetics; Isoantigens/metabolism; Isoantigens/pharmacology; Male; Mice; Microscopy, Confocal; Molecular Sequence Data; Muramidase/metabolism; Ovum/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/pharmacology; Seminal Plasma Proteins/genetics; Seminal Plasma Proteins/metabolism; Seminal Plasma Proteins/pharmacology; Sequence Alignment; Sequence Analysis, DNA; Sperm-Ovum Interactions/drug effects; Sperm-Ovum Interactions/genetics; Sperm-Ovum Interactions/physiology; Spermatozoa/metabolism
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