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PMID:15935059

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Citation

Zhu, J, Apparsundaram, S, Bardo, MT and Dwoskin, LP (2005) Environmental enrichment decreases cell surface expression of the dopamine transporter in rat medial prefrontal cortex. J. Neurochem. 93:1434-43

Abstract

Rats raised in an enriched environmental condition (EC) exhibit a decreased (35%) maximal velocity (V(max)) of [3H]dopamine (DA) uptake in medial prefrontal cortex (mPFC) compared with rats raised in an impoverished condition (IC); however, no differences between EC and IC groups in V(max) for [3H]DA uptake were found in nucleus accumbens and striatum. Using biotinylation and immunoblotting techniques, the present study examined whether the brain region-specific decrease in DA transporter (DAT) function is the result of a reduction in DAT cell surface expression. In mPFC, nucleus accumbens and striatum, total DAT immunoreactivity was not different between EC and IC groups. Whereas no differences in cell surface expression of DAT were found in nucleus accumbens and striatum, DAT immunoreactivity in the biotinylated cell surface fraction of mPFC was decreased (39%) in EC compared with IC rats, consistent with the magnitude of the previously observed decrease in V(max) for [3H]DA uptake in mPFC in EC rats. These results suggest that the decrease in DAT cell surface expression in the mPFC may be responsible for decreased DAT function in the mPFC of EC compared with IC rats, and that there is plasticity in the regulatory mechanisms mediating DAT trafficking and function.

Links

PubMed Online version:10.1111/j.1471-4159.2005.03130.x

Keywords

Animals; Cell Membrane/metabolism; Corpus Striatum/metabolism; Dopamine/metabolism; Dopamine Plasma Membrane Transport Proteins; Down-Regulation/physiology; Environment Design; Male; Membrane Glycoproteins/metabolism; Membrane Transport Proteins/metabolism; Nerve Tissue Proteins/metabolism; Neuronal Plasticity/physiology; Neurons/metabolism; Nucleus Accumbens/metabolism; Physical Stimulation; Prefrontal Cortex/metabolism; Protein Transport/physiology; Rats; Rats, Sprague-Dawley; Sensory Deprivation/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

AGRFC:DHAA

GO:0018786: haloalkane dehalogenase activity

ECO:0000314:

F

Substrate specificity profile of DatA with the set of 36 chlorinated (in white), iodinated (in gray), and brominated (in black) substrates. Specific activity of DatA was measured using the Iwasaki method. From this data it is shown that 1,3 dibromopropane as most active when in the presence of Data. Indicated that DatA possesses substrate specificity similar to those of DmbC and DbeA. This helps identify its function.

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See also

References

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