PMID:15914229

Citation	Zhang, X, Bourhis, JM, Longhi, S, Carsillo, T, Buccellato, M, Morin, B, Canard, B and Oglesbee, M (2005) Hsp72 recognizes a P binding motif in the measles virus N protein C-terminus. Virology 337:162-74
Abstract	The major inducible 70-kDa heat shock protein (hsp72) binds measles virus (MV) nucleocapsids and increases MV gene expression. The cytoplasmic tail of the MV N protein (N(TAIL)) contains three hydrophobic domains (Box-1-3) that are potential targets of hsp72 interaction. Low affinity binding to Box-3 is correlated to hsp72-dependent stimulation of MV minireplicon reporter gene expression whereas interactions between hsp72 and Box-1 and/or -2 have not been documented. The present work showed that virus deficient in Box-3/hsp72 interaction retains the ability to form nucleocapsid/hsp72 complexes, identifying Box-2 but not Box-1 as a mediator of high affinity hsp72 binding. Box-2 is the binding site for the viral P protein X domain (XD), where P tethers the viral polymerase to nucleocapsid in support of transcription and genome replication, and competitive inhibition of XD binding to N(TAIL) by hsp72 was shown. Recognition of a common binding site by P and hsp72 represents a potential mechanism for host cell modulation of viral gene expression.
Links	PubMed Online version:10.1016/j.virol.2005.03.035
Keywords	Amino Acid Motifs; Binding Sites; Cell Line; HSP72 Heat-Shock Proteins; Heat-Shock Proteins/metabolism; Humans/metabolism; Measles virus/chemistry; Nucleocapsid Proteins/chemistry; Nucleocapsid Proteins/metabolism; Protein Binding; Virus Replication
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MEASF:NCAP	enables	GO:0030544: Hsp70 protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:P54652	F		Seeded From UniProt
MEASF:NCAP	involved_in	GO:0050434: positive regulation of viral transcription	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt
MEASF:NCAP	involved_in	GO:0039656: modulation by virus of host gene expression	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt
MEASF:NCAP	part_of	GO:0019013: viral nucleocapsid	ECO:0000315: mutant phenotype evidence used in manual assertion		C		Seeded From UniProt
MEASF:NCAP	part_of	GO:0039643: host cell viral nucleoid	ECO:0000315: mutant phenotype evidence used in manual assertion		C		Seeded From UniProt

References

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