GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15883197
| Citation |
Chen, H, Cohen, DM, Choudhury, DM, Kioka, N and Craig, SW (2005) Spatial distribution and functional significance of activated vinculin in living cells. J. Cell Biol. 169:459-70 |
|---|---|
| Abstract |
Conformational change is believed to be important to vinculin's function at sites of cell adhesion. However, nothing is known about vinculin's conformation in living cells. Using a Forster resonance energy transfer probe that reports on changes in vinculin's conformation, we find that vinculin is in the actin-binding conformation in a peripheral band of adhesive puncta in spreading cells. However, in fully spread cells with established polarity, vinculin's conformation is variable at focal adhesions. Time-lapse imaging reveals a gradient of conformational change that precedes loss of vinculin from focal adhesions in retracting regions. At stable or protruding regions, recruitment of vinculin is not necessarily coupled to the actin-binding conformation. However, a different measure of vinculin conformation, the recruitment of vinexin beta by activated vinculin, shows that autoinhibition of endogenous vinculin is relaxed at focal adhesions. Beyond providing direct evidence that vinculin is activated at focal adhesions, this study shows that the specific functional conformation correlates with regional cellular dynamics. |
| Links |
PubMed PMC2171941 Online version:10.1083/jcb.200410100 |
| Keywords |
Actins/metabolism; Adaptor Proteins, Signal Transducing/metabolism; Animals; Antigens, Bacterial/pharmacology; Bacterial Proteins/pharmacology; Cell Adhesion/physiology; Cell Line; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cell Shape/physiology; Cell Surface Extensions/metabolism; Cell Surface Extensions/ultrastructure; Focal Adhesions/metabolism; Focal Adhesions/ultrastructure; Humans; Mice; Microscopy, Video; Muscle Proteins/metabolism; Protein Binding/drug effects; Protein Binding/physiology; Protein Conformation; Vinculin/chemistry; Vinculin/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
