PMID:15883194

Citation	Fukata, Y, Tzingounis, AV, Trinidad, JC, Fukata, M, Burlingame, AL, Nicoll, RA and Bredt, DS (2005) Molecular constituents of neuronal AMPA receptors. J. Cell Biol. 169:399-404
Abstract	Dynamic regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immunoprecipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory proteins (TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope-tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs.
Links	PubMed PMC2171936 Online version:10.1083/jcb.200501121
Keywords	Animals; Brain/metabolism; Calcium Channels/metabolism; Cell Line; Guanylate Kinase; Heat-Shock Proteins/metabolism; Humans; Intracellular Signaling Peptides and Proteins; Lymphokines/metabolism; Membrane Proteins/metabolism; Mice; Mice, Transgenic; Molecular Chaperones/metabolism; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Oocytes; Receptors, AMPA/genetics; Receptors, AMPA/metabolism; Synaptic Membranes/metabolism; Xenopus
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.