GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15817223

From GONUTS
Jump to: navigation, search
Citation

Elworthy, S, Pinto, JP, Pettifer, A, Cancela, ML and Kelsh, RN (2005) Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent. Mech. Dev. 122:659-69

Abstract

Zebrafish lacking functional sox10 have defects in non-ectomesenchymal neural crest derivatives including the enteric nervous system (ENS) and as such provide an animal model for human Waardenburg Syndrome IV. Here, we characterize zebrafish phox2b as a functionally conserved marker of the developing ENS. We show that morpholino-mediated knockdown of Phox2b generates fish modeling Hirschsprung disease. Using markers, including phox2b, we investigate the ontogeny of the sox10 ENS phenotype. As previously shown for melanophore development, ENS progenitor fate specification fails in these mutant fish. However, in addition, we trace back the sox10 mutant ENS defect to an even earlier time point, finding that most neural crest cells fail to migrate ventrally to the gut primordium.

Links

PubMed Online version:10.1016/j.mod.2004.12.008

Keywords

Amino Acid Sequence; Animals; Carrier Proteins/genetics; Carrier Proteins/metabolism; Carrier Proteins/physiology; Central Nervous System/embryology; Chromosome Mapping; Enteric Nervous System/embryology; Gene Expression Regulation, Developmental; High Mobility Group Proteins/genetics; High Mobility Group Proteins/metabolism; High Mobility Group Proteins/physiology; Homeodomain Proteins/metabolism; Homeodomain Proteins/physiology; In Situ Hybridization; Melanophores/metabolism; Microscopy, Fluorescence; Models, Genetic; Molecular Sequence Data; Mutation; Neural Crest/metabolism; Neurons/metabolism; Phenotype; Phylogeny; SOXE Transcription Factors; Sequence Homology, Amino Acid; Stem Cells/metabolism; Time Factors; Transcription Factors/metabolism; Transcription Factors/physiology; Zebrafish; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism; Zebrafish Proteins/physiology

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.