PMID:15814844

Citation	Burri, L, Strahm, Y, Hawkins, CJ, Gentle, IE, Puryer, MA, Verhagen, A, Callus, B, Vaux, D and Lithgow, T (2005) Mature DIABLO/Smac is produced by the IMP protease complex on the mitochondrial inner membrane. Mol. Biol. Cell 16:2926-33
Abstract	DIABLO/Smac is a mitochondrial protein that can promote apoptosis by promoting the release and activation of caspases. To do so, DIABLO/Smac must first be processed by a mitochondrial protease and then released into the cytosol, and we show this in an intact cellular system. We propose that the precursor form of DIABLO/Smac enters the mitochondria through a stop-transfer pathway and is processed to its active form by the inner membrane peptidase (IMP) complex. Catalytic subunits of the mammalian IMP complex were identified based on sequence conservation and functional complementation, and the novel sequence motif RX(5)P in Imp1 and NX(5)S in Imp2 distinguish the two catalytic subunits. DIABLO/Smac is one of only a few specific proteins identified as substrates for the IMP complex in the mitochondrial intermembrane space.
Links	PubMed PMC1142436 Online version:10.1091/mbc.E04-12-1086
Keywords	Amino Acid Motifs; Amino Acid Sequence; Blotting, Western; Catalytic Domain; Conserved Sequence; Endopeptidases/chemistry; Endopeptidases/metabolism; Green Fluorescent Proteins/metabolism; Intracellular Membranes/enzymology; Intracellular Membranes/metabolism; Microscopy, Confocal; Mitochondria/enzymology; Mitochondrial Proteins/biosynthesis; Models, Biological; Molecular Sequence Data; Mutation; Protein Structure, Tertiary; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins/biosynthesis; Sequence Homology, Amino Acid; Substrate Specificity
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