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PMID:15809310
| Citation |
Luo, Y and Radice, GL (2005) N-cadherin acts upstream of VE-cadherin in controlling vascular morphogenesis. J. Cell Biol. 169:29-34 |
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| Abstract |
Endothelial cells express two classic cadherins, VE-cadherin and N-cadherin. The importance of VE-cadherin in vascular development is well known; however, the function of N-cadherin in endothelial cells remains poorly understood. Contrary to previous studies, we found that N-cadherin localizes to endothelial cell-cell junctions in addition to its well-known diffusive membrane expression. To investigate the role of N-cadherin in vascular development, N-cadherin was specifically deleted from endothelial cells in mice. Loss of N-cadherin in endothelial cells results in embryonic lethality at mid-gestation due to severe vascular defects. Intriguingly, loss of N-cadherin caused a significant decrease in VE-cadherin and its cytoplasmic binding partner, p120ctn. The down-regulation of both VE-cadherin and p120ctn was confirmed in cultured endothelial cells using small interfering RNA to knockdown N-cadherin. We also show that N-cadherin is important for endothelial cell proliferation and motility. These findings provide a novel paradigm by which N-cadherin regulates angiogenesis, in part, by controlling VE-cadherin expression at the cell membrane. |
| Links |
PubMed PMC2171890 Online version:10.1083/jcb.200411127 |
| Keywords |
Animals; Antigens, CD; Cadherins/genetics; Cadherins/metabolism; Catenins; Cell Adhesion Molecules/metabolism; Cell Membrane/metabolism; Cell Movement/genetics; Cell Movement/physiology; Cell Proliferation; Cells, Cultured; Embryo, Mammalian/metabolism; Embryo, Mammalian/pathology; Endothelium, Vascular/cytology; Endothelium, Vascular/embryology; Endothelium, Vascular/metabolism; Gene Expression Regulation, Developmental/genetics; Gene Expression Regulation, Developmental/physiology; Mice; Morphogenesis/genetics; Morphogenesis/physiology; Phosphoproteins/metabolism; RNA Interference; Umbilical Veins/cytology; Umbilical Veins/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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