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PMID:15800061
| Citation |
Gatfield, J, Albrecht, I, Zanolari, B, Steinmetz, MO and Pieters, J (2005) Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules. Mol. Biol. Cell 16:2786-98 |
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| Abstract |
Coronin 1 is a member of the coronin protein family specifically expressed in leukocytes and accumulates at sites of rearrangements of the F-actin cytoskeleton. Here, we describe that coronin 1 molecules are coiled coil-mediated homotrimeric complexes, which associate with the plasma membrane and with the cytoskeleton via two distinct domains. Association with the cytoskeleton was mediated by trimerization of a stretch of positively charged residues within a linker region between the N-terminal, WD repeat-containing domain and the C-terminal coiled coil. In contrast, neither the coiled coil nor the positively charged residues within the linker domain were required for plasma membrane binding, suggesting that the N-terminal, WD repeat-containing domain mediates membrane interaction. The capacity of coronin 1 to link the leukocyte cytoskeleton to the plasma membrane may serve to integrate outside-inside signaling with modulation of the cytoskeleton. |
| Links |
PubMed PMC1142424 Online version:10.1091/mbc.E05-01-0042 |
| Keywords |
Actins/metabolism; Amino Acid Sequence; Animals; Cell Line; Cell Membrane/metabolism; Circular Dichroism; Cytoskeleton/metabolism; Electrophoresis, Gel, Two-Dimensional; Humans; Immunohistochemistry; Introns; Jurkat Cells; Leukocytes/cytology; Macrophages/cytology; Mice; Microfilament Proteins/chemistry; Microfilament Proteins/genetics; Microfilament Proteins/isolation & purification; Microfilament Proteins/metabolism; Microfilament Proteins/ultrastructure; Models, Biological; Molecular Sequence Data; Protein Isoforms/chemistry; Protein Isoforms/genetics; Protein Isoforms/metabolism; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Spectrophotometry, Ultraviolet; Subcellular Fractions/metabolism |
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Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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