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PMID:15765519
| Citation |
Lu, S, Borst, DE and Horowits, R (2005) N-RAP expression during mouse heart development. Dev. Dyn. 233:201-12 |
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| Abstract |
N-RAP gene expression and N-RAP localization were studied during mouse heart development using semiquantitative reverse transcriptase-polymerase chain reaction and immunofluorescence. N-RAP mRNA was detected at embryonic day (E) 10.5, significantly increased from E10.5 to E16.5, and remained essentially constant from E16.5 until 21 days after birth. In E9.5-10.5 heart tissue, N-RAP protein was primarily associated with developing premyofibril structures containing alpha-actinin, as well as with the Z-lines and M-lines of more-mature myofibrils. In contrast, N-cadherin was concentrated in patches at the periphery of the cardiomyocytes. N-RAP labeling markedly increased between E10.5 and E16.5; almost all of the up-regulated N-RAP was associated with intercalated disk structures, and the proportion of mature sarcomeres containing N-RAP decreased. In adult hearts, specific N-RAP staining was only observed at the intercalated disks and was not found in the sarcomeres. The results are consistent with N-RAP functioning as a catalytic scaffolding molecule, with low levels of the scaffold being sufficient to repetitively catalyze key steps in myofibril assembly. |
| Links |
PubMed Online version:10.1002/dvdy.20314 |
| Keywords |
Actinin/genetics; Actinin/metabolism; Animals; Cadherins/genetics; Cadherins/metabolism; Heart/embryology; Heart/physiology; Mice; Muscle Proteins/genetics; Muscle Proteins/metabolism; Organ Specificity; RNA, Messenger/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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