PMID:15741318

Citation	Isono, K, Mizutani-Koseki, Y, Komori, T, Schmidt-Zachmann, MS and Koseki, H (2005) Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1. Genes Dev. 19:536-41
Abstract	Polycomb group (PcG) proteins are responsible for the stable repression of homeotic (Hox) genes by forming multimeric protein complexes. We show (1) physical interaction between components of the U2 small nuclear ribonucleoprotein particle (U2 snRNP), including Sf3b1 and PcG proteins Zfp144 and Rnf2; and (2) that Sf3b1 heterozygous mice exhibit skeletal transformations concomitant with ectopic Hox expressions. These alterations are enhanced by Zfp144 mutation but repressed by Mll mutation (a trithorax-group gene). Importantly, the levels of Sf3b1 in PcG complexes were decreased in Sf3b1-heterozygous embryos. These findings suggest that Sf3b1-PcG protein interaction is essential for true PcG-mediated repression of Hox genes.
Links	PubMed PMC551574 Online version:10.1101/gad.1284605
Keywords	Animals; Bone and Bones/embryology; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Down-Regulation/genetics; Down-Regulation/physiology; Gene Expression Regulation, Developmental/genetics; Gene Expression Regulation, Developmental/physiology; Homeodomain Proteins/biosynthesis; Homeodomain Proteins/genetics; Mice; Mice, Knockout; Mutation; Myeloid-Lymphoid Leukemia Protein; Phosphoproteins/metabolism; Protein Binding/genetics; Protein Binding/physiology; Proto-Oncogenes/genetics; Repressor Proteins; Ribonucleoprotein, U2 Small Nuclear/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Two-Hybrid System Techniques
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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