PMID:15733871

Citation	Park, CY, Lee, JH, Yoo, JH, Moon, BC, Choi, MS, Kang, YH, Lee, SM, Kim, HS, Kang, KY, Chung, WS, Lim, CO and Cho, MJ (2005) WRKY group IId transcription factors interact with calmodulin. FEBS Lett. 579:1545-50
Abstract	Calmodulin (CaM) is a ubiquitous Ca(2+)-binding protein known to regulate diverse cellular functions by modulating the activity of various target proteins. We isolated a cDNA encoding AtWRKY7, a novel CaM-binding transcription factor, from an Arabidopsis expression library with horseradish peroxidase-conjugated CaM. CaM binds specifically to the Ca(2+)-dependent CaM-binding domain (CaMBD) of AtWRKY7, as shown by site-directed mutagenesis, a gel mobility shift assay, a split-ubiquitin assay, and a competition assay using a Ca2+/CaM-dependent enzyme. Furthermore, we show that the CaMBD of AtWRKY7 is a conserved structural motif (C-motif) found in group IId of the WRKY protein family.
Links	PubMed Online version:10.1016/j.febslet.2005.01.057
Keywords	Amino Acid Sequence; Arabidopsis/chemistry; Arabidopsis/classification; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/isolation & purification; Arabidopsis Proteins/metabolism; Calmodulin/metabolism; Calmodulin-Binding Proteins/chemistry; Calmodulin-Binding Proteins/classification; Calmodulin-Binding Proteins/genetics; Calmodulin-Binding Proteins/metabolism; Molecular Sequence Data; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Phylogeny; Protein Binding; Sequence Alignment; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/isolation & purification; Transcription Factors/metabolism; Ubiquitin/metabolism
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