PMID:15721260

Citation	Takata, H, Tanaka, Y and Matsuura, A (2005) Late S phase-specific recruitment of Mre11 complex triggers hierarchical assembly of telomere replication proteins in Saccharomyces cerevisiae. Mol. Cell 17:573-83
Abstract	In Saccharomyces cerevisiae, telomere replication occurs in late S phase and is accompanied by dynamic remodeling of its protein components. Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S phase. MRX is required for the late S phase-specific recruitment of ATR-like kinase Mec1 to the telomeres. Mec1, in turn, contributes to the assembly of the telomerase regulators Cdc13 and Est1 at the telomere ends. Our results provide a model for the hierarchical assembly of telomere-replication proteins in late S phase; this involves triggering by the loading of MRX onto the chromosome termini. The recruitment of DNA repair-related proteins to the telomeres at particular times in the cell cycle suggests that the normal terminus of a chromosome is recognized as a DSB during the course of replication.
Links	PubMed Online version:10.1016/j.molcel.2005.01.014
Keywords	Chromatin Immunoprecipitation; DNA/genetics; DNA Repair; DNA Replication; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Endodeoxyribonucleases/genetics; Endodeoxyribonucleases/metabolism; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Intracellular Signaling Peptides and Proteins; Phenotype; Protein-Serine-Threonine Kinases; S Phase; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Telomere/genetics; Telomere/metabolism
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